Behrens’ Fixative

Water	1	L
Cupric chloride	2	g
Cupric nitrate	2	g
Phenol	10	g
Acetic acid, glacial	10	mL

Fixation time should be determined by trial.

Copper fixatives are not often used in modern histology. They have a reputation for good preservation, as the copper present may act as a mordant for some dyes, including hematoxylin. For that reason nuclear staining may be enhanced.

 

Reference

Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Mayer, (1920)
    Zoomikrotechnik
    Berlin, Germany