Other Adhesives

Sometimes strange procedures are recommended.

Hollande
Garlic cloves
Water
Chloroform
50 g
80 mL
1 mL
Crush the garlic cloves in a pestle and morter. Add the water and chloroform and grind together to extract as much material as possible. Leave 24 hours, then filter.
This is a pectin solution that is claimed to be quite effective, although I would presume it is somewhat aromatic. Spread a small amount thinly on a clean, grease free slide, pick up from a waterbath and bake on as usual. Compare with amylopectin.

Presumably, commercial pectin solutions (Certo and others in the USA) used for making jams and jellies could also be used in a similar fashion, although a preservative may need to be added to them. Remember that starch based materials may be oxidisable to aldehydes so that any staining method which demonstrates aldehydes will give background staining.

 

Reference
Gray, Peter. (1954)
The Microtomist's Formulary and Guide.
Originally published by:– The Blakiston Co.
Republished by:– Robert E. Krieger Publishing Co.
  Citing:–
    Hollande (1911)
    Archives d'anatomie microscopique. v. 13, p. 171.
    Paris, France.

 


 
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