The coloured anion of eosin Y, and sometimes that of eosin B, are referred to as eosinol. They may be sold under the name eosin Y, spirit soluble and eosin B, spirit soluble. Eosin Y and eosin B themselves are the sodium salts of these anions.
Compare the formulae of the eosinols above to that of fluorescein.
Although eosinol is used to refer to the eosin Y anion primarily, the anion of many of the eosin homologues probably could be made in a similar fashion.
To use eosinol, simply add some to either a dehydrating ethanol or a xylene, until the fluid is adequately red. Some trial and error is needed to establish the correct amount. Prestain the nuclei with an alum hematoxylin, and blue. Then allow the sections to remain in the coloured fluid until stained darkly enough. Again, some trial and error is needed to determine the correct time. The dye is most valuable for difficult to stain tissues such as those from fetuses.
Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA
Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.
Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.