Toluidine Blue O

Property Data
Common name
Suggested name
Other names
C.I. number
C.I. name
Class
Ionisation
Solubility aqueous
Solubility ethanol
Absorption maximum
 
 
 
Colour
Empirical formula
Formula weight
Toluidine blue
Toluidine blue O
Tolonium chloride
52040
Basic blue 17
Thiazin
Basic
3.82%
0.57%
620-622 (Conn)
632 (Gurr)
640.4 (Merck)
626 (Aldrich)
Blue
C15H16N3SCl
305.8

Toluidine blue O is a metachromatic dye, and is frequently employed in that capacity. It is a blue nuclear counterstain, and can be used to demonstrate Nissl substance. It is useful for staining mast cell granules, both in metachromatic and orthochromatic techniques. Compare its formula to other methylene blue homologues.

 

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA

 


 

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