Nile Blue A

Nile blue A

Nile red

Property Data Data
Common name
Suggested name
Other names
C.I. number
C.I. name
Class
Ionisation
Solubility aqueous
Solubility ethanol
Absorption maximum
 
 
Colour
Empirical formula
Formula weight
Nile blue
Nile blue A
Nile blue sulphate
51180
Basic blue 12
Oxazin
Basic
Soluble

635-645 (Conn)
637 (Gurr)
633 & 638 (Aldrich)
Blue
C20H20N3OCl
353.9
Nile red
Nile red



Oxazone
Lysochrome

Soluble
553 (Aldrich)
 
 
Red
C20H18N2O2
318.4

Nile blue A forms a red staining lysochrome, named nile red, when it is boiled with dilute sulphuric acid. During this treatment, oxygen is substituted for an amino group converting it to the other dye. It is from this ability that nile blue A gains its value in histology. The red lysochrome remains dissolved in aqueous mixture with Nile blue A, but will preferentially stain lipids from the solution, colouring them red. The Nile blue, in turn, stains the nuclei and background blue in contrast.

 

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.

 


 

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