Celestin blue B

Property Data
Common name
Suggested name
Other names
C.I. number
C.I. name
Class
Ionisation
Solubility aqueous
Solubility ethanol
Absorption maximum
 
Colour
Empirical formula
Formula weight
Celestin blue
Celestin blue B
Coelestin blue
51050
Mordant blue 14
Oxazin
Basic
2%
1.5%
654.5, 600 (Conn)
652 (Gurr)
Blue
C17H18N3O4Cl
363.8

Celestin blue B is used in solution with an iron alum mordant as a hematoxylin substitute in the H&E stain. When this staining is followed by a progressive alum hematoxylin, an acid resistant nuclear stain is obtained (celestin blue-hemalum sequence).

 

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

 


 

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