Safranin O

Dimethyl safranin

Trimethyl safranin

Property Data
Common name
Suggested name
Other names
C.I. number
C.I. name
Class
Ionisation
Solubility aqueous
Solubility ethanol
Absorption maximum
 
Colour
Empirical formula
 
Formula weight
 
Safranin
Safranin O

50240
Basic red 2
Safranin
Basic
5.45%
3.41%
530 (Conn, Aldrich)
517 (Gurr)
Red
C20H19N4Cl (Dimethyl)
C21H21N4Cl (Trimethyl)
350.8 (Dimethyl)
364.9 (Trimethyl)

Safranin O is a mixture of the two compounds shown, according to Conn. Aldrich and Gurr give only the dimethyl compound.

Safranin O is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. For tissue sections it is useful as a red nuclear counterstain, and was popular at one time for staining cartilage metachromatically yellow, against red nuclei on a pink background.

 

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.

 


 

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