Acridine Orange

Property Data Common name Suggested name Other names C.I. number C.I. name Class Ionisation Solubility aqueous Solubility ethanol Absorption maximum     Colour Empirical formula Formula weight Acridine orange Acridine orange – 46005 Basic orange 14 Acridine Basic Readily Moderate 497, 467 (Conn) 489 (Aldrich) 539 (Gurr) Yellow C17H20N3Cl 301.8

Acridine orange has been recommended as a fluorochrome to differentiate between DNA (green fluorescence) and RNA (red fluorescence). It also makes a good fluorescent Schiff reagent.

 

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.