This dye is not commonly used in North America, although it is a perfectly satisfactory substitute for Eosin Y ws. In Europe it is used instead of Phloxine in the hematoxylin phloxine saffron (HPS) stain, which is the same as the hematoxylin erythrosine saffron (HES) method in Europe.
Compare the structural formulae of some eosin homologues.
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.
Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.
Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA
Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.