Eosin Y

Property Data
Common name
Suggested name
Other names
 
 
C.I. number
C.I. name
Class
Ionisation
Solubility aqueous
Solubility ethanol
Absorption maximum
 
 
Colour
Empirical formula
Formula weight
Eosin Y
Eosin Y ws
Eosine Yellowish
Bromoeosine
Tetrabromofluorescein
45380
Acid red 87
Fluorone
Acid
40%
2%
515-518 (Conn)
517 (Gurr)
514 (Aldrich)
Red
C20H6O5Br4Na2
691.9

Eosin Y is the most common counterstain to alum hematoxylin in the Hematoxylin and Eosin method (H&E). It stains satisfactorily from both aqueous and ethanolic solution. It is one of the dyes in Papanicolaou's EA solutions for staining exfoliative cytology for cervical cancer screening. It can be used to make Romanowsky stains. It is widely used in numerous procedures. It is strongly fluorescent, but this property is hardly ever used.

The free radical, eosinol Y is made by treating the dye with hydrochloric acid. It is sometimes called eosin Y, spirit soluble. Eosinol Y is soluble in ethanol and slightly soluble in xylene, but not in water, and is useful for staining difficult tissues.

 

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.

 


 

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