Fluorescein

Fluorescein

Uranin

Fluorescein
isothiocyanate

Property Data Data
Common name
Suggested name
Other names
C.I. number
C.I. name
Class
Ionisation
Solubility aqueous
Solubility ethanol
Absorption maximum
 
Colour
Empirical formula
Formula weight
Fluorescein
Fluorescein

45350
Solvent yellow 94
Fluorone
Acid
Slight
2.21%
493.5, 460
485 (Gurr)
Yellow
C20H12O5
332.3
Uranin
Uranin

45350
Acid yellow 73
Fluorone
Acid
50%
7.19%
493.5 (Conn)
485 (Gurr)
Yellow
C20H10Na2O5
376.3

Fluorescein is the precursor of the group of dyes collectively referred to as the eosins, but has little use itself in light microscopy even though it is strongly fluorescent. It is used to detect contamination in water supplies because of the intense fluorescence at low concentration. The closely related fluorescein isothiocyanate, is attached to antibodies and used as a fluorescent marker in immunofluorescence microscopy.

Uranin is the sodium salt of fluorescein.

 

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.

Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

 


 

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