Rhodamine B

Pyronon B
according to Conn

Pyronin B
according to Merck

Property Data
Common name
Suggested name
Other names
C.I. number
C.I. name
Class
Ionisation
Solubility aqueous
Solubility ethanol
Absorption maximum
 
Colour
Empirical formula
Formula weight
 Rhodamine B
Rhodamine B
–
45170
Basic violet 10
Rhodamine
Basic
Very
Very
556.5 (Conn), 548 (Gurr)
543, 355 (Aldrich)
Red
C28H31N2O3Cl
479

Rhodamine B is an amphoteric dye, although usually listed as basic as it has an overall positive charge. It is most commonly used as a fluorochrome, an example being in mixture with auramine O to demonstrate acid fast organisms.

The structural formula given in the Merck Index is slightly different than in Conn, having methyl groups instead of ethyl groups.

 

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.

Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA

 


 
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Last updated May 2005.