Stainsfile - Pyronin Y

Property	Data
Common name Suggested name Other names C.I. number C.I. name Class Ionisation Solubility aqueous Solubility ethanol Absorption maximum Colour Empirical formula Formula weight	Pyronin Y Pyronin Y Pyronin G 45005 – Pyronin Basic 8.96% 0.6% 545 (Gurr) 548 (Aldrich) 552 (Conn) Red C₁₇H₁₉N₂OCl 302.8

Pyronin Y is used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna-Pappenheim method.

Compare the formula with other pyronins.

Reference
R. D. Lillie.
Conn's Biological Stains
Williams & Wilkins, Baltimore, MD., U.S.A.

Aldrich chemical catalogue, 1992
Aldrich Chemical Company, Milwaukee, WI, USA.

Susan Budavari, Editor, (1996)
The Merck Index, Ed. 12
Merck & Co., Inc., Whitehouse Station, NJ, USA

Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.

Last updated May 2005.