There has been little use of this dye in histology. Lendrum, Slidders and Fraser suggest it as a yellow fibre stain in their trichrome technique.
Due to the way this dye is made, it does not have a single structural formula, but numerous closely related compounds are collectively referred to as CI 4000, direct yellow 11. The formula above should be considered a general type rather than a specification.
Edward Gurr, (1971)
Synthetic dyes in biology, medicine and chemistry
Academic Press, London, England.
Colour Index, Ed. 2. (1956)
Society of dyers and colourists, UK.